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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.

Anti-ACKR3 Antibody

HPA032003
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
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Product group Antibodies
TargetACKR3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACKR3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57007
  • Target name
    ACKR3
  • Target description
    atypical chemokine receptor 3
  • Target synonyms
    atypical chemokine receptor 3; chemokine (C-X-C motif) receptor 7; chemokine orphan receptor 1; CMKOR1; C-X-C chemokine receptor type 7; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog; RDC1; RDC-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25106
  • Protein Name
    Atypical chemokine receptor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GPCR RDC1/CXCR-7/ACKR3 using anti-GPCR RDC1/CXCR-7/ACKR3 antibody (A02656-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR RDC1/CXCR-7/ACKR3 antigen affinity purified polyclonal antibody (Catalog # A02656-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR RDC1/CXCR-7/ACKR3 at approximately 41 kDa. The expected band size for GPCR RDC1/CXCR-7/ACKR3 is at 41 kDa.
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