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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, hela cells, Primary antibody: CSB-PA240234(ACP2 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, hela cells, Primary antibody: CSB-PA240234(ACP2 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: 293T cells, hela cells, Primary antibody: CSB-PA240234(ACP2 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes

ACP2 Antibody

CSB-PA240234
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetACP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID53
  • Target name
    ACP2
  • Target description
    acid phosphatase 2, lysosomal
  • Target synonyms
    LAP; lysosomal acid phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11117
  • Protein Name
    Lysosomal acid phosphatase
  • Scientific Description
    This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Mutations in this gene or in the related alpha subunit gene cause acid phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACP2 using anti-ACP2 antibody (A06554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACP2 antigen affinity purified polyclonal antibody (Catalog # A06554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACP2 at approximately 76 kDa. The expected band size for ACP2 is at 76 kDa.
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