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ACSS2 / ACAS2 Antibody (clone OTI3C6)

LS-C173346
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetACSS2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ACSS2 / ACAS2 Antibody (clone OTI3C6)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3C6
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55902
  • Target name
    ACSS2
  • Target description
    acyl-CoA synthetase short chain family member 2
  • Target synonyms
    ACAS2; ACECS; AceCS1; acetate thiokinase; acetate-CoA ligase; acetyl-CoA synthetase 1; acetyl-Coenzyme A synthetase 2 (ADP forming); acetyl-coenzyme A synthetase, cytoplasmic; ACS; ACSA; acyl-activating enzyme; cytoplasmic acetyl-coenzyme A synthetase; dJ1161H23.1; propionate--CoA ligase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACSS2 using anti-ACSS2 antibody (A02809-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 antigen affinity purified polyclonal antibody (Catalog # A02809-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2 at approximately 79 kDa. The expected band size for ACSS2 is at 79 kDa.
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