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Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ACTR2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ACTR2 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ACTR2 Polyclonal Antibody at dilution 1:40

ACTR2 Polyclonal Antibody

E-AB-14618
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ACTR2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (A03898-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse HEPA1-6 wholel cell lysates, Lane 4: mouse SP2/0 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human PC-3 whole cell lysates, Lane 9: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arp2/ACTR2 antigen affinity purified polyclonal antibody (Catalog # A03898-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arp2/ACTR2 at approximately 45KD. The expected band size for Arp2/ACTR2 is at 45KD.
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