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Western blot All lanes: ACVR1 antibody at 6ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
Western blot All lanes: ACVR1 antibody at 6ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
Western blot All lanes: ACVR1 antibody at 6ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa

ACVR1 Antibody

CSB-PA001257ESR2HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetACVR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACVR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID90
  • Target name
    ACVR1
  • Target description
    activin A receptor type 1
  • Target synonyms
    ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI, activin receptor type-1, TGF-B superfamily receptor type I, activin A receptor, type I, activin A receptor, type II-like kinase 2, activin receptor type I, activin receptor-like kinase 2, hydroxyalkyl-protein kinase, serine/threonine-protein kinase receptor R1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04771
  • Protein Name
    Activin receptor type-1
  • Scientific Description
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis .
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACVR1 using anti-ACVR1 antibody (M00798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR1 antigen affinity purified monoclonal antibody (Catalog # M00798) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR1 at approximately 57 kDa. The expected band size for ACVR1 is at 57 kDa.
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