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Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.

Anti-ACVR1 Antibody

HPA007505
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACVR1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ACVR1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID90
  • Target name
    ACVR1
  • Target description
    activin A receptor type 1
  • Target synonyms
    activin A receptor, type I; activin A receptor, type II-like kinase 2; activin receptor type I; activin receptor type-1; activin receptor-like kinase 2; ACTRI; ACVR1A; ACVRLK2; ALK2; FOP; hydroxyalkyl-protein kinase; serine/threonine-protein kinase receptor R1; SKR1; TGF-B superfamily receptor type I; TSRI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04771
  • Protein Name
    Activin receptor type-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ACVR1 using anti-ACVR1 antibody (M00798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR1 antigen affinity purified monoclonal antibody (Catalog # M00798) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR1 at approximately 57 kDa. The expected band size for ACVR1 is at 57 kDa.
Product group Antibodies
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Western blot All lanes: ACVR1 antibody at 6ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
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