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Adam8 Polyclonal Antibody

CAC11385
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetADAM8
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Overview

  • Supplier
    Biomatik
  • Product Name
    Adam8 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID101
  • Target name
    ADAM8
  • Target description
    ADAM metallopeptidase domain 8
  • Target synonyms
    CD156, CD156a, MS2, disintegrin and metalloproteinase domain-containing protein 8, a disintegrin and metalloproteinase domain 8, cell surface antigen MS2, human leukocyte differentiation antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78325
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 8
  • Scientific Description
    The Adam8 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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A431 (Positive) and HepG2 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody (bs-4195R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Western Blot Positive WB detected in: Raji whole cell lysate, THP-1 whole cell lysate, RAW264.7 whole cell lysate All lanes: ADAM8 antibody at 3.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 79, 81 kDa Observed band size: 89 kDa
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Figure 1. Western blot analysis of MS2/ADAM8 using anti-MS2/ADAM8 antibody (A04306-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MS2/ADAM8 antigen affinity purified polyclonal antibody (Catalog # A04306-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MS2/ADAM8 at approximately 80 kDa. The expected band size for MS2/ADAM8 is at 80 kDa.
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