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Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Anti-ADAM8 Antibody

HPA064637
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetADAM8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ADAM8 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID101
  • Target name
    ADAM8
  • Target description
    ADAM metallopeptidase domain 8
  • Target synonyms
    CD156, CD156a, MS2, disintegrin and metalloproteinase domain-containing protein 8, a disintegrin and metalloproteinase domain 8, cell surface antigen MS2, human leukocyte differentiation antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78325
  • Protein Name
    Disintegrin and metalloproteinase domain-containing protein 8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MS2/ADAM8 using anti-MS2/ADAM8 antibody (A04306-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MS2/ADAM8 antigen affinity purified polyclonal antibody (Catalog # A04306-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MS2/ADAM8 at approximately 80 kDa. The expected band size for MS2/ADAM8 is at 80 kDa.
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A431 (Positive) and HepG2 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody (bs-4195R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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