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ProductsBack/Goat anti-MS2 / ADAM8 / CD156

Goat anti-MS2 / ADAM8 / CD156

EB05399
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetADAM8
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-MS2 / ADAM8 / CD156 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID101
  • Target name
    ADAM8
  • Target description
    ADAM metallopeptidase domain 8
  • Target synonyms
    CD156, CD156a, MS2, disintegrin and metalloproteinase domain-containing protein 8, a disintegrin and metalloproteinase domain 8, cell surface antigen MS2, human leukocyte differentiation antigen
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001100.3; NP_001157962.1. GeneIDs all Nonhuman: 11501 (mouse);. Purification: Antigen affinity purified. Names and symbols: ADAM8; CD156; a disintegrin and metalloproteinase domain 8; CD156 MS2; ADAM metallopeptidase domain 8; MGC134985; MS2; cell surface antigen MS2; disintegrin and metalloproteinase domain-containing protein 8; human leukocyte differentiation antigen
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MS2/ADAM8 using anti-MS2/ADAM8 antibody (A04306-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MS2/ADAM8 antigen affinity purified polyclonal antibody (Catalog # A04306-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MS2/ADAM8 at approximately 80 kDa. The expected band size for MS2/ADAM8 is at 80 kDa.
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