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Lane 1: Recombinant protein lysates probed with ADAMTS4 Monoclonal Antibody, Unconjugated (bsm-51704M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant protein lysates probed with ADAMTS4 Monoclonal Antibody, Unconjugated (bsm-51704M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant protein lysates probed with ADAMTS4 Monoclonal Antibody, Unconjugated (bsm-51704M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

ADAMTS4 Monoclonal Antibody

BSM-51704M
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetADAMTS4
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Overview

  • Supplier
    Bioss
  • Product Name
    ADAMTS4 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:1000-4000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D4F8
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID9507
  • Target name
    ADAMTS4
  • Target description
    ADAM metallopeptidase with thrombospondin type 1 motif 4
  • Target synonyms
    ADAMTS-2, ADAMTS-4, ADMP-1, A disintegrin and metalloproteinase with thrombospondin motifs 4, ADAM-TS 4, ADAM-TS4, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4, aggrecanase-1, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Protein IDO75173
  • Protein Name
    A disintegrin and metalloproteinase with thrombospondin motifs 4
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAMTS4 using anti-ADAMTS4 antibody (A02899). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human ADAMTS4 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS4 antigen affinity purified polyclonal antibody (Catalog # A02899) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS4 at approximately 58KD. The expected band size for ADAMTS4 is at 53KD.
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