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ADAMTS5 Antibody

LS-C408561
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetADAMTS5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ADAMTS5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 101kDa, while the observed MW by Western blot was 105kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 101kDa, while the observed MW by Western blot was 105kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11096
  • Target name
    ADAMTS5
  • Target description
    ADAM metallopeptidase with thrombospondin type 1 motif 5
  • Target synonyms
    ADAM-TS 11, ADAM-TS 5, ADAM-TS5, ADAMTS-11, ADAMTS-5, ADAMTS11, ADMP-2, A disintegrin and metalloproteinase with thrombospondin motifs 5, a disintegrin and metalloproteinase with thrombospondin motifs 11, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2), aggrecanase-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADAMTS5 using anti-ADAMTS5 antibody (A02802-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human SGC-7901 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse testis tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS5 antigen affinity purified polyclonal antibody (Catalog # A02802-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS5 at approximately 75 kDa. The expected band size for ADAMTS5 is at 102 kDa.
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