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ADAP / FYB Antibody

LS-C812972
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetFYB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ADAP / FYB Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2533
  • Target name
    FYB1
  • Target description
    FYN binding protein 1
  • Target synonyms
    ADAP; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130; FYN-binding protein 1; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLAP-130; SLP-76-associated phosphoprotein; THC3
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FYB using anti-FYB antibody (M32410-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FYB antigen affinity purified monoclonal antibody (Catalog # M32410-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FYB at approximately 120 kDa. The expected band size for FYB is at 85 kDa.
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