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Goat anti-FYB

EB08254
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFYB1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-FYB Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2533
  • Target name
    FYB1
  • Target description
    FYN binding protein 1
  • Target synonyms
    ADAP, FYB, PRO0823, SLAP-130, SLAP130, THC3, FYN-binding protein 1, FYB-120/130, FYN-T-binding protein, SLP-76-associated phosphoprotein, adhesion and degranulation-promoting adaptor protein, p120/p130
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001456.3; NP_955367.1; NP_001230022.1. Purification: Antigen affinity purified. Names and symbols: FYB; FYN binding protein (FYB-120/130) ; ADAP; PRO0823; SLAP-130; FYN-binding protein (FYB-120/130); adhesion and degranulation-promoting adaptor protein
  • Reactivity Supplier
    Human, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FYB using anti-FYB antibody (M32410-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FYB antigen affinity purified monoclonal antibody (Catalog # M32410-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FYB at approximately 120 kDa. The expected band size for FYB is at 85 kDa.
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