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Western Blot analysis of COLO205 cells using Fyb Polyclonal Antibody
Western Blot analysis of COLO205 cells using Fyb Polyclonal Antibody
Western Blot analysis of COLO205 cells using Fyb Polyclonal Antibody

FYB Antibody

CSB-PA002589
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFYB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FYB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2533
  • Target name
    FYB1
  • Target description
    FYN binding protein 1
  • Target synonyms
    ADAP, FYB, PRO0823, SLAP-130, SLAP130, THC3, FYN-binding protein 1, FYB-120/130, FYN-T-binding protein, SLP-76-associated phosphoprotein, adhesion and degranulation-promoting adaptor protein, p120/p130
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15117
  • Protein Name
    FYN-binding protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FYB using anti-FYB antibody (M32410-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FYB antigen affinity purified monoclonal antibody (Catalog # M32410-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FYB at approximately 120 kDa. The expected band size for FYB is at 85 kDa.
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