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Western Blot analysis of HepG2 cells using ADCK4 Polyclonal Antibody
Western Blot analysis of HepG2 cells using ADCK4 Polyclonal Antibody
Western Blot analysis of HepG2 cells using ADCK4 Polyclonal Antibody

ADCK4 Antibody

CSB-PA000823
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCOQ8B
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADCK4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79934
  • Target name
    COQ8B
  • Target description
    coenzyme Q8B
  • Target synonyms
    aarF domain containing kinase 4; aarF domain-containing protein kinase 4; ADCK4; atypical kinase COQ8B, mitochondrial; coenzyme Q protein 8B; NPHS9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96D53
  • Protein Name
    Atypical kinase COQ8B, mitochondrial
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COQ8B using anti-COQ8B antibody (A32263-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COQ8B antigen affinity purified polyclonal antibody (Catalog # A32263-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COQ8B at approximately 65 kDa. The expected band size for COQ8B is at 60 kDa.
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