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HepG2 cells were stained with ADCK4 Polyclonal Antibody, Unconjugated (bs-8070R) at 1:200 and incubated for 90 minutesat room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were stained with ADCK4 Polyclonal Antibody, Unconjugated (bs-8070R) at 1:200 and incubated for 90 minutesat room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HepG2 cells were stained with ADCK4 Polyclonal Antibody, Unconjugated (bs-8070R) at 1:200 and incubated for 90 minutesat room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

ADCK4 Polyclonal Antibody

BS-8070R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCOQ8B
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ADCK4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID79934
  • Target name
    COQ8B
  • Target description
    coenzyme Q8B
  • Target synonyms
    aarF domain containing kinase 4; aarF domain-containing protein kinase 4; ADCK4; atypical kinase COQ8B, mitochondrial; coenzyme Q protein 8B; NPHS9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96D53
  • Protein Name
    Atypical kinase COQ8B, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COQ8B using anti-COQ8B antibody (A32263-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COQ8B antigen affinity purified polyclonal antibody (Catalog # A32263-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COQ8B at approximately 65 kDa. The expected band size for COQ8B is at 60 kDa.
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