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ProductsBack/Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI18C7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.

Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI18C7]

CF500323
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAK1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI18C7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI18C7
  • Gene ID203
  • Target name
    AK1
  • Target description
    adenylate kinase 1
  • Target synonyms
    ADK, Adk1, CNSHA3, HTL-S-58j, adenylate kinase isoenzyme 1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, adenylate monophosphate kinase, epididymis secretory sperm binding protein, myokinase, testis secretory sperm binding protein Li 58j
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP00568
  • Protein Name
    Adenylate kinase isoenzyme 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) AK1 mouse monoclonal antibody, clone OTI18C7 (formerly 18C7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of AK1 using anti-AK1 antibody (PB10033). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AK1 antigen affinity purified polyclonal antibody (Catalog # PB10033) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AK1 at approximately 22 kDa. The expected band size for AK1 is at 22 kDa.
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