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Western Blot Positive WB detected in: Rat liver tissue All lanes: ADH5 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western Blot Positive WB detected in: Rat liver tissue All lanes: ADH5 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western Blot Positive WB detected in: Rat liver tissue All lanes: ADH5 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa

ADH5 Antibody

CSB-PA001357LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetADH5
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADH5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID128
  • Target name
    ADH5
  • Target description
    alcohol dehydrogenase 5 (class III), chi polypeptide
  • Target synonyms
    ADH-3; ADHX; alcohol dehydrogenase (class III), chi polypeptide; alcohol dehydrogenase class chi chain; alcohol dehydrogenase class-3; alcohol dehydrogenase class-III; AMEDS; BMFS7; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; GSNOR; HEL-S-60p; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11766
  • Protein Name
    Alcohol dehydrogenase class-3
  • Scientific Description
    Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADH5 using anti-ADH5 antibody (RP1111). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MOLT4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH5 antigen affinity purified polyclonal antibody (Catalog # RP1111) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH5 at approximately 39 kDa. The expected band size for ADH5 is at 40 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ADH5 Polyclonal Antibody, Unconjugated (bs-12448R) at 1:200 overnight at 4°C, followed by secondary antibody DAB and counterstaining.
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