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Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Anti-ADH5 Antibody

HPA044578
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetADH5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ADH5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID128
  • Target name
    ADH5
  • Target description
    alcohol dehydrogenase 5 (class III), chi polypeptide
  • Target synonyms
    ADH-3, ADHX, AMEDS, BMFS7, FALDH, FDH, GSH-FDH, GSNOR, HEL-S-60p, alcohol dehydrogenase class-3, S-(hydroxymethyl)glutathione dehydrogenase, S-nitrosoglutathione reductase, alcohol dehydrogenase (class III), chi polypeptide, alcohol dehydrogenase class chi chain, alcohol dehydrogenase class-III, epididymis secretory sperm binding protein Li 60p, formaldehyde dehydrogenase, glutathione-dependent formaldehyde dehydrogenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11766
  • Protein Name
    Alcohol dehydrogenase class-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ADH5 using anti-ADH5 antibody (RP1111). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MOLT4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH5 antigen affinity purified polyclonal antibody (Catalog # RP1111) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH5 at approximately 39 kDa. The expected band size for ADH5 is at 40 kDa.
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