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Goat anti-ADH5

EB07612
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetADH5
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ADH5 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID128
  • Target name
    ADH5
  • Target description
    alcohol dehydrogenase 5 (class III), chi polypeptide
  • Target synonyms
    ADH-3; ADHX; alcohol dehydrogenase (class III), chi polypeptide; alcohol dehydrogenase class chi chain; alcohol dehydrogenase class-3; alcohol dehydrogenase class-III; AMEDS; BMFS7; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; GSNOR; HEL-S-60p; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000662.3. GeneIDs all Nonhuman: 11532 (mouse);. Purification: Antigen affinity purified. Names and symbols: ADH5; alcohol dehydrogenase 5 (class III), chi polypeptide; ADH-3; ADHX; FDH ; Alcohol dehydrogenase (class III), chi polypeptide; class III alcohol dehydrogenase 5 chi subunit; formaldehyde dehydrogenase; glutathione-dependent formaldehyde dehydrogenase;
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ADH5 using anti-ADH5 antibody (RP1111). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MOLT4 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH5 antigen affinity purified polyclonal antibody (Catalog # RP1111) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH5 at approximately 39 kDa. The expected band size for ADH5 is at 40 kDa.
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