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ADRA1A antibody

70R-30682
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    ADRA1A antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate All lanes: ADRA1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 53, 48, 51, 33, 38, 36, 41 kDa Observed band size: 52 kDa
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Figure 1. Western blot analysis of ADRA1A using anti-ADRA1A antibody (PB9752). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Rat Liver Tissue Lysate at 50ug, Lane 4: Mouse Liver Tissue Lysate at 50ug, Lane 5: Mouse Lung Tissue Lysate at 50ug, Lane 6: 22RV1 Whole Cell Lysate at 40ug, Lane 7: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified polyclonal antibody (Catalog # PB9752) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa.
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