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ADRA1A Antibody (Internal)

LS-C368567
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetADRA1A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ADRA1A Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:500), IF, WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID148
  • Target name
    ADRA1A
  • Target description
    adrenoceptor alpha 1A
  • Target synonyms
    ADRA1C, ADRA1L1, ALPHA1AAR, alpha-1A adrenergic receptor, G protein coupled receptor, alpha-1A adrenoceptor, alpha-1A adrenoreceptor, alpha-1C adrenergic receptor
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Heart tissue lysates; Lane 2: Rat Heart tissue lysates probed with ADRA1A Polyclonal Antibody, Unconjugated (bs-0600R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of ADRA1A using anti-ADRA1A antibody (PB9752). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Rat Liver Tissue Lysate at 50ug, Lane 4: Mouse Liver Tissue Lysate at 50ug, Lane 5: Mouse Lung Tissue Lysate at 50ug, Lane 6: 22RV1 Whole Cell Lysate at 40ug, Lane 7: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified polyclonal antibody (Catalog # PB9752) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa.
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