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Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate All lanes: ADRA1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 53, 48, 51, 33, 38, 36, 41 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate All lanes: ADRA1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 53, 48, 51, 33, 38, 36, 41 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate All lanes: ADRA1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 53, 48, 51, 33, 38, 36, 41 kDa Observed band size: 52 kDa

ADRA1A Antibody

CSB-PA001385PA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetADRA1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    ADRA1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID148
  • Target name
    ADRA1A
  • Target description
    adrenoceptor alpha 1A
  • Target synonyms
    ADRA1C, ADRA1L1, ALPHA1AAR, alpha-1A adrenergic receptor, G protein coupled receptor, alpha-1A adrenoceptor, alpha-1A adrenoreceptor, alpha-1C adrenergic receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35348
  • Protein Name
    Alpha-1A adrenergic receptor
  • Scientific Description
    This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ADRA1A using anti-ADRA1A antibody (PB9752). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Rat Liver Tissue Lysate at 50ug, Lane 4: Mouse Liver Tissue Lysate at 50ug, Lane 5: Mouse Lung Tissue Lysate at 50ug, Lane 6: 22RV1 Whole Cell Lysate at 40ug, Lane 7: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified polyclonal antibody (Catalog # PB9752) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa.
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