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WB analysis of HeLa cell lysate using GTX31462 AFAP antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of HeLa cell lysate using GTX31462 AFAP antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of HeLa cell lysate using GTX31462 AFAP antibody. Working concentration : (A) 1 and (B) 2 microg/ml

AFAP antibody

GTX31462
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAFAP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AFAP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID60312
  • Target name
    AFAP1
  • Target description
    actin filament associated protein 1
  • Target synonyms
    AFAP, AFAP-110, AFAP110, actin filament-associated protein 1, 110 kDa actin filament-associated protein, actin filament-associated protein, 110 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N556
  • Protein Name
    Actin filament-associated protein 1
  • Scientific Description
    The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AFAP/AFAP1 using anti-AFAP/AFAP1 antibody (A05258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat stomach tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFAP/AFAP1 antigen affinity purified polyclonal antibody (Catalog # A05258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFAP/AFAP1 at approximately 120KD. The expected band size for AFAP/AFAP1 is at 120KD.
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