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AFAP1 Antibody

CAB3478
Assay Genie
TargetAFAP1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    AFAP1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID60312
  • Target name
    AFAP1
  • Target description
    actin filament associated protein 1
  • Target synonyms
    110 kDa actin filament-associated protein; actin filament-associated protein 1; actin filament-associated protein, 110 kDa; AFAP; AFAP110; AFAP-110
  • Host
    Rabbit
  • Protein IDQ8N556
  • Protein Name
    Actin filament-associated protein 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AFAP/AFAP1 using anti-AFAP/AFAP1 antibody (A05258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat stomach tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFAP/AFAP1 antigen affinity purified polyclonal antibody (Catalog # A05258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFAP/AFAP1 at approximately 120KD. The expected band size for AFAP/AFAP1 is at 120KD.
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Immunohistochemistry analysis in human colon and liver tissues using HPA015642 antibody. Corresponding AFAP1 RNA-seq data are presented for the same tissues.
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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA098902(AFAP1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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