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WB analysis of various sample lysates using GTX32996 AHCYL1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32996 AHCYL1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32996 AHCYL1 antibody. Dilution : 1:1000 Loading : 25microg per lane

AHCYL1 antibody

GTX32996
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAHCYL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AHCYL1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10768
  • Target name
    AHCYL1
  • Target description
    adenosylhomocysteinase like 1
  • Target synonyms
    adenosylhomocysteinase 2; adoHcyase 2; DCAL; DC-expressed AHCY-like molecule; dendritic cell expressed AHCY-like protein; epididymis secretory sperm binding protein; inositol 1,4,5-trisphosphate receptor-binding protein; IP(3)Rs binding protein released with IP(3); IRBIT; PPP1R78; PRO0233; protein phosphatase 1, regulatory subunit 78; putative adenosylhomocysteinase 2; S-adenosyl homocysteine hydrolase homolog; S-adenosylhomocysteine hydrolase-like protein 1; S-adenosyl-L-homocysteine hydrolase 2; XPVKONA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43865
  • Protein Name
    S-adenosylhomocysteine hydrolase-like protein 1
  • Scientific Description
    The protein encoded by this gene interacts with inositol 1,4,5-trisphosphate receptor, type 1 and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: AHCYL1 antibody at 12ug/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59, 54 kDa Observed band size: 59 kDa
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Figure 1. Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (A08908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBIT/AHCYL1 antigen affinity purified polyclonal antibody (Catalog # A08908-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 61 kDa. The expected band size for IRBIT/AHCYL1 is at 61 kDa.
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