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AHCYL1 / DCAL Antibody

LS-C815846
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAHCYL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AHCYL1 / DCAL Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500), IHC-P (1:200 - 1:1000), IP, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10768
  • Target name
    AHCYL1
  • Target description
    adenosylhomocysteinase like 1
  • Target synonyms
    adenosylhomocysteinase 2; adoHcyase 2; DCAL; DC-expressed AHCY-like molecule; dendritic cell expressed AHCY-like protein; epididymis secretory sperm binding protein; inositol 1,4,5-trisphosphate receptor-binding protein; IP(3)Rs binding protein released with IP(3); IRBIT; PPP1R78; PRO0233; protein phosphatase 1, regulatory subunit 78; putative adenosylhomocysteinase 2; S-adenosyl homocysteine hydrolase homolog; S-adenosylhomocysteine hydrolase-like protein 1; S-adenosyl-L-homocysteine hydrolase 2; XPVKONA
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRBIT/AHCYL1 using anti-IRBIT/AHCYL1 antibody (A08908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRBIT/AHCYL1 antigen affinity purified polyclonal antibody (Catalog # A08908-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRBIT/AHCYL1 at approximately 61 kDa. The expected band size for IRBIT/AHCYL1 is at 61 kDa.
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