More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/AIDA Antibody
Immunofluorescence staining of U251 cells with CSB-PA839294LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of U251 cells with CSB-PA839294LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of U251 cells with CSB-PA839294LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

AIDA Antibody

CSB-PA839294LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetAIDA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    AIDA Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64853
  • Target name
    AIDA
  • Target description
    axin interactor, dorsalization associated
  • Target synonyms
    axin interaction partner and dorsalization antagonist; axin interactor, dorsalization-associated protein; C1orf80
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96BJ3
  • Protein Name
    Axin interactor, dorsalization-associated protein
  • Scientific Description
    Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Related products

Product group Antibodies
Bethyl Laboratories
Rabbit anti-AIDA Antibody, Affinity PurifiedA303-302A
ApplicationsImmunoPrecipitation, Western Blot
TargetAIDA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
AIDA Antibody (aa100-150)LS-C289988
ApplicationsImmunoPrecipitation, Western Blot
TargetAIDA
  • SizePrice
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Product group Antibodies
Atlas Antibodies
Anti-AIDA AntibodyHPA027935
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetAIDA
  • SizePrice
Figure 1. Western blot analysis of AIDA using anti-AIDA antibody (A04989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIDA antigen affinity purified polyclonal antibody (Catalog # A04989-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIDA at approximately 36 kDa. The expected band size for AIDA is at 35 kDa.
Product group Antibodies
Boster Bio
Anti-AIDA Antibody Picoband(r)A04989-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetAIDA
  • SizePrice
Product group Antibodies
Assay Genie
AIDA AntibodyPACO63839
ApplicationsImmunoFluorescence, ELISA
TargetAIDA
  • SizePrice