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AIDA Antibody

PACO63839
Assay Genie
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetAIDA
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Overview

  • Supplier
    Assay Genie
  • Product Name
    AIDA Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID64853
  • Target name
    AIDA
  • Target description
    axin interactor, dorsalization associated
  • Target synonyms
    C1orf80, axin interactor, dorsalization-associated protein, axin interaction partner and dorsalization antagonist
  • Host
    Rabbit
  • Protein IDQ96BJ3
  • Protein Name
    Axin interactor, dorsalization-associated protein
  • Scientific Description
    AIDA Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of AIDA using anti-AIDA antibody (A04989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIDA antigen affinity purified polyclonal antibody (Catalog # A04989-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIDA at approximately 36 kDa. The expected band size for AIDA is at 35 kDa.
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