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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

Anti-AIDA Antibody

HPA027935
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetAIDA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AIDA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64853
  • Target name
    AIDA
  • Target description
    axin interactor, dorsalization associated
  • Target synonyms
    axin interaction partner and dorsalization antagonist; axin interactor, dorsalization-associated protein; C1orf80
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96BJ3
  • Protein Name
    Axin interactor, dorsalization-associated protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Immunofluorescence staining of U251 cells with CSB-PA839294LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of AIDA using anti-AIDA antibody (A04989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIDA antigen affinity purified polyclonal antibody (Catalog # A04989-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIDA at approximately 36 kDa. The expected band size for AIDA is at 35 kDa.
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