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Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.
Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.
Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.

AIF1/Iba1 Polyclonal Antibody

BS-1363R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAIF1
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Overview

  • Supplier
    Bioss
  • Product Name
    AIF1/Iba1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor 1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Liao CF, Chen CC, Lu YW, et al. Effects of endogenous inflammation signals elicited by nerve growth factor, interferon-γ, and interleukin-4 on peripheral nerve regeneration. J Biol Eng. 2019,13:86. doi: 10.1186/s13036-019-0216-x
    Read this paper
  • Yue J, Zhang C, Shi X, et al. Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex. Brain Dev. 2019,41(10):829-838. doi: 10.1016/j.braindev.2019.08.002
    Read this paper
  • Ko CH, Shie MY, Lin JH, et al. Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats. Sci Rep. 2017,7(1):17489. doi: 10.1038/s41598-017-17792-2
    Read this paper
  • Kouam AF, Yuan F, Njayou FN, et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Front Pharmacol. 2017,8:653. doi: 10.3389/fphar.2017.00653
    Read this paper
  • Hu Y, Kodithuwakku ND, Zhou L, et al. Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway. Molecules. 2017,22(6). doi: 10.3390/molecules22060937
    Read this paper
  • Fu J, Xia X, Liu Z, et al. The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning. Toxicology. 2017,381:39-50. doi: 10.1016/j.tox.2017.02.015
    Read this paper
  • Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017,30(1):79-107. doi: 10.1293/tox.2016-0047
    Read this paper
  • Hsu ST, Yao CH, Hsu YM, et al. Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model. Sci Rep. 2017,7:42280. doi: 10.1038/srep42280
    Read this paper
  • Ji YB, Zhuang PP, Ji Z, et al. TFP5 peptide, derived from CDK5-activating cofactor p35, provides neuroprotection in early-stage of adult ischemic stroke. Sci Rep. 2017,7:40013. doi: 10.1038/srep40013
    Read this paper

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Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
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