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Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.
Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.
Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.

AIF1/Iba1 Polyclonal Antibody

BS-1363R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetAIF1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    AIF1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1; allograft inflammatory factor 1; IBA1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; IRT1; IRT-1; protein G1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Effects of endogenous inflammation signals elicited by nerve growth factor, interferon-gamma, and interleukin-4 on peripheral nerve regeneration. Liao CF et al., 2019, J Biol Eng
    Read more
  • Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex. Yue J et al., 2019 Nov, Brain Dev
    Read more
  • Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats. Ko CH et al., 2017 Dec 13, Sci Rep
    Read more
  • Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Kouam AF et al., 2017, Front Pharmacol
    Read more
  • Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway. Hu Y et al., 2017 Jun 6, Molecules
    Read more
  • The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning. Fu J et al., 2017 Apr 15, Toxicology
    Read more
  • Databases for technical aspects of immunohistochemistry. Furukawa S et al., 2017 Jan, J Toxicol Pathol
    Read more
  • Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model. Hsu ST et al., 2017 Feb 9, Sci Rep
    Read more
  • TFP5 peptide, derived from CDK5-activating cofactor p35, provides neuroprotection in early-stage of adult ischemic stroke. Ji YB et al., 2017 Jan 3, Sci Rep
    Read more

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Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
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