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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in microglia.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in microglia.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in microglia.

Anti-AIF1 Antibody

AMAB91671
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAIF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AIF1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL10930
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1; allograft inflammatory factor 1; IBA1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; IRT1; IRT-1; protein G1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
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