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Anti-AIF1 [GT1-mAb1]

AB03986-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetAIF1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-AIF1 [GT1-mAb1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1-mAb1
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1; allograft inflammatory factor 1; IBA1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; IRT1; IRT-1; protein G1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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