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AIMP2 Antibody

LS-C763935
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetAIMP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AIMP2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7965
  • Target name
    AIMP2
  • Target description
    aminoacyl tRNA synthetase complex interacting multifunctional protein 2
  • Target synonyms
    aminoacyl tRNA synthase complex-interacting multifunctional protein 2; ARS-interacting multi-functional protein 2; HLD17; JTV1; JTV-1; multisynthase complex auxiliary component p38; multisynthetase complex auxiliary component p38; P38; protein JTV-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: Jurkat cell lysates, Lane 6: CEM cell lysates, Lane 7: HUT cell lysates, Lane 8: U93T cell lysates, Lane 9: U93T cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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