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Immunohistochemical staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.

Anti-AIMP2 Antibody

HPA019098
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAIMP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AIMP2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7965
  • Target name
    AIMP2
  • Target description
    aminoacyl tRNA synthetase complex interacting multifunctional protein 2
  • Target synonyms
    aminoacyl tRNA synthase complex-interacting multifunctional protein 2; ARS-interacting multi-functional protein 2; HLD17; JTV1; JTV-1; multisynthase complex auxiliary component p38; multisynthetase complex auxiliary component p38; P38; protein JTV-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13155
  • Protein Name
    Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti AIMP2 Polyclonal Antibody, Unconjugated (bs-2509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.
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Figure 1. Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: Jurkat cell lysates, Lane 6: CEM cell lysates, Lane 7: HUT cell lysates, Lane 8: U93T cell lysates, Lane 9: U93T cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA151818(AIMP2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
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ReactivityHuman, Mouse, Rat
TargetAIMP2
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Protein IDQ13155
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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
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ReactivityHuman
TargetAIMP2
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