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ProductsBack/AIMP2 Polyclonal Antibody
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti AIMP2 Polyclonal Antibody, Unconjugated (bs-2509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti AIMP2 Polyclonal Antibody, Unconjugated (bs-2509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti AIMP2 Polyclonal Antibody, Unconjugated (bs-2509R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.

AIMP2 Polyclonal Antibody

BS-2509R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rabbit, Rat
TargetAIMP2
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Overview

  • Supplier
    Bioss
  • Product Name
    AIMP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7965
  • Target name
    AIMP2
  • Target description
    aminoacyl tRNA synthetase complex interacting multifunctional protein 2
  • Target synonyms
    HLD17, JTV-1, JTV1, P38, aminoacyl tRNA synthase complex-interacting multifunctional protein 2, ARS-interacting multi-functional protein 2, multisynthase complex auxiliary component p38, multisynthetase complex auxiliary component p38, protein JTV-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13155
  • Protein Name
    Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Du W, Su J, Ye D, et al. Pinellia ternata Attenuates Mucus Secretion and Airway Inflammation after Inhaled Corticosteroid Withdrawal in COPD Rats. Am J Chin Med. 2016,44(5):1027-41. doi: 10.1142/S0192415X16500579
    Read this paper

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Figure 1. Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: Jurkat cell lysates, Lane 6: CEM cell lysates, Lane 7: HUT cell lysates, Lane 8: U93T cell lysates, Lane 9: U93T cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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