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AKAP14 Antibody (aa5-54)

LS-C200111
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAKAP14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    AKAP14 Antibody (aa5-54)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 22 kDa
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Peptide-ELISA (1:10000), WB (1:500 - 1:1000) Western Blot: Predicted MW 22 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID158798
  • Target name
    AKAP14
  • Target description
    A-kinase anchoring protein 14
  • Target synonyms
    AKAP28, PRKA14, A-kinase anchor protein 14, A kinase (PRKA) anchor protein 14, A-kinase anchor protein 28 kDa, A-kinase anchoring protein 28, AKAP 28, AKAP-14, protein kinase A-anchoring protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKAP14 using anti-AKAP14 antibody (M15886). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP14 antigen affinity purified monoclonal antibody (Catalog # M15886) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP14 at approximately 21,23 kDa. The expected band size for AKAP14 is at 21 kDa.
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