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Western Blot analysis of MCF7 cells using AKAP 95 Polyclonal Antibody
Western Blot analysis of MCF7 cells using AKAP 95 Polyclonal Antibody
Western Blot analysis of MCF7 cells using AKAP 95 Polyclonal Antibody

AKAP8 Antibody

CSB-PA000845
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAKAP8
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Overview

  • Supplier
    Cusabio
  • Product Name
    AKAP8 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10270
  • Target name
    AKAP8
  • Target description
    A-kinase anchoring protein 8
  • Target synonyms
    A kinase (PRKA) anchor protein 8; AKAP 95; AKAP-8; AKAP95; AKAP-95; A-kinase anchor protein 8; A-kinase anchor protein, 95kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43823
  • Protein Name
    A-kinase anchor protein 8
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (A05133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP 95/AKAP8 antigen affinity purified polyclonal antibody (Catalog # A05133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP 95/AKAP8 at approximately 92 kDa. The expected band size for AKAP 95/AKAP8 is at 76 kDa.
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