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Anti-AKAP8 Antibody

144-03049
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAKAP8
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-AKAP8 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10270
  • Target name
    AKAP8
  • Target description
    A-kinase anchoring protein 8
  • Target synonyms
    A kinase (PRKA) anchor protein 8; AKAP 95; AKAP-8; AKAP95; AKAP-95; A-kinase anchor protein 8; A-kinase anchor protein, 95kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43823
  • Protein Name
    A-kinase anchor protein 8
  • Scientific Description
    AKAP8 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (A05133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP 95/AKAP8 antigen affinity purified polyclonal antibody (Catalog # A05133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP 95/AKAP8 at approximately 92 kDa. The expected band size for AKAP 95/AKAP8 is at 76 kDa.
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