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AKAP95 Antibody (YA2696, Rabbit)

HY-P82951
MedChem Express
TargetAKAP8
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    AKAP95 Antibody (YA2696, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10270
  • Target name
    AKAP8
  • Target description
    A-kinase anchoring protein 8
  • Target synonyms
    AKAP 95, AKAP-8, AKAP-95, AKAP95, A-kinase anchor protein 8, A kinase (PRKA) anchor protein 8, A-kinase anchor protein, 95kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    AKAP95 Antibody (YA2696) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2696), targeting AKAP95, with a predicted molecular weight of 76 kDa (observed band size: 95 kDa). AKAP95 Antibody (YA2696) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (A05133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP 95/AKAP8 antigen affinity purified polyclonal antibody (Catalog # A05133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP 95/AKAP8 at approximately 92 kDa. The expected band size for AKAP 95/AKAP8 is at 76 kDa.
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