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WB analysis of various sample lysates using GTX30040 AKR1B1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30040 AKR1B1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX30040 AKR1B1 antibody. Dilution : 1:1000 Loading : 25microg per lane

AKR1B1 antibody

GTX30040
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetAKR1B1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AKR1B1 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID231
  • Target name
    AKR1B1
  • Target description
    aldo-keto reductase family 1 member B
  • Target synonyms
    ADR; aldehyde reductase 1; aldo-keto reductase family 1 member B1; aldose reductase; ALDR1; ALR2; AR; Lii5-2 CTCL tumor antigen; low Km aldose reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15121
  • Protein Name
    Aldose reductase
  • Scientific Description
    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Protection of 6-OHDA neurotoxicity by PGF2alpha through FP-ERK-Nrf2 signaling in SH-SY5Y cells. Sano A et al., 2021 Feb 28, Toxicology
    Read more

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Figure 1. Western blot analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1B1 antigen affinity purified polyclonal antibody (Catalog # PB10035) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1B1 at approximately 36 kDa. The expected band size for AKR1B1 is at 36 kDa.
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