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ProductsBack/AKR1B1 antibody [N1C3]

AKR1B1 antibody [N1C3]

GTX113381
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAKR1B1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AKR1B1 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.06 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID231
  • Target name
    AKR1B1
  • Target description
    aldo-keto reductase family 1 member B
  • Target synonyms
    ADR; aldehyde reductase 1; aldo-keto reductase family 1 member B1; aldose reductase; ALDR1; ALR2; AR; Lii5-2 CTCL tumor antigen; low Km aldose reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15121
  • Protein Name
    Aldose reductase
  • Scientific Description
    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • 3H-1,2-Dithiole-3-Thione Protects Lens Epithelial Cells against Fructose-Induced Epithelial-Mesenchymal Transition via Activation of AMPK to Eliminate AKR1B1-Induced Oxidative Stress in Diabetes Mellitus. Wu TT et al., 2021 Jul 6, Antioxidants (Basel)
    Read more
  • Fluid Intake Restriction Concomitant to Sweetened Beverages Hydration Induce Kidney Damage. Garcia-Arroyo FE et al., 2020, Oxid Med Cell Longev
    Read more
  • Targeting NPL4 via drug repositioning using disulfiram for the treatment of clear cell renal cell carcinoma. Yoshino H et al., 2020, PLoS One
    Read more
  • AKR1B1-Induced Epithelial-Mesenchymal Transition Mediated by RAGE-Oxidative Stress in Diabetic Cataract Lens. Wu TT et al., 2020 Mar 25, Antioxidants (Basel)
    Read more
  • A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose. Garcia-Arroyo FE et al., 2019 Nov 16, Int J Mol Sci
    Read more
  • Dicarbonyl stress and glyoxalase enzyme system regulation in human skeletal muscle. Mey JT et al., 2018 Feb 1, Am J Physiol Regul Integr Comp Physiol
    Read more
  • Fructose Synthesis and Transport at the Uterine-Placental Interface of Pigs: Cell-Specific Localization of SLC2A5, SLC2A8, and Components of the Polyol Pathway. Steinhauser CB et al., 2016 Nov, Biol Reprod
    Read more
  • Rehydration with soft drink-like beverages exacerbates dehydration and worsens dehydration-associated renal injury. Garcia-Arroyo FE et al., 2016 Jul 1, Am J Physiol Regul Integr Comp Physiol
    Read more
  • Sorbitol dehydrogenase overexpression and other aspects of dysregulated protein expression in human precancerous colorectal neoplasms: a quantitative proteomics study. Uzozie A et al., 2014 May, Mol Cell Proteomics
    Read more

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Figure 1. Western blot analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1B1 antigen affinity purified polyclonal antibody (Catalog # PB10035) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1B1 at approximately 36 kDa. The expected band size for AKR1B1 is at 36 kDa.
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