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WB analysis of HepG2 lysate (33ug per lane) using AKR1C1 antibody at the indicated dilutions.
WB analysis of HepG2 lysate (33ug per lane) using AKR1C1 antibody at the indicated dilutions.
WB analysis of HepG2 lysate (33ug per lane) using AKR1C1 antibody at the indicated dilutions.

AKR1C1 antibody [AT6D10]

GTX53684
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAKR1C1
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Overview

  • Supplier
    GeneTex
  • Product Name
    AKR1C1 antibody [AT6D10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT6D10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1645
  • Target name
    AKR1C1
  • Target description
    aldo-keto reductase family 1 member C1
  • Target synonyms
    20 alpha-hydroxysteroid dehydrogenase; 20-ALPHA-HSD; 2-ALPHA-HSD; aldo-keto reductase C; aldo-keto reductase family 1 member C1; C9; chlordecone reductase homolog HAKRC; DD1; DD1/DD2; DDH; DDH1; dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; H-37; HAKRC; HBAB; hepatic dihydrodiol dehydrogenase; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; MBAB; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ04828
  • Protein Name
    Aldo-keto reductase family 1 member C1
  • Scientific Description
    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Transcriptional consequences of XPA disruption in human cell lines. Manandhar M et al., 2017 Sep, DNA Repair (Amst)
    Read more

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Figure 1. Western blot analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mosue liver tissue lysates, Lane 7: mosue kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C1/C2 antigen affinity purified polyclonal antibody (Catalog # PB10036) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1C1/C2 at approximately 37 kDa. The expected band size for AKR1C1/C2 is at 37 kDa.
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