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Western blot analysis of extracts of various cell lines using AKR1C1 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using AKR1C1 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using AKR1C1 Polyclonal Antibody at dilution of 1:3000.

AKR1C1 Polyclonal Antibody

E-AB-64412
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AKR1C1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HCCP tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mosue liver tissue lysates, Lane 7: mosue kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C1/C2 antigen affinity purified polyclonal antibody (Catalog # PB10036) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1C1/C2 at approximately 37 kDa. The expected band size for AKR1C1/C2 is at 37 kDa.
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Immunofluorescence staining of HepG2 cells with CSB-PA001542LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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