WB of Mouse anti-AKT pT308 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT pT308 antibody at a 1:4,000 dilution. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) (p/n 610-1303) was used at a 1:40,000 dilution for 1 h at 4°C. Block: 3% BSA (p/n BSA-30) in TBS for 30 min at RT.