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Western blot All lanes: Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody at 8ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 99, 88, 100, 56 kDa Observed band size: 99 kDa
Western blot All lanes: Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody at 8ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 99, 88, 100, 56 kDa Observed band size: 99 kDa
Western blot All lanes: Cytosolic 10-formyltetrahydrofolate dehydrogenase antibody at 8ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse gonad tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 99, 88, 100, 56 kDa Observed band size: 99 kDa

ALDH1L1 Antibody

CSB-PA001569ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetALDH1L1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDH1L1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10840
  • Target name
    ALDH1L1
  • Target description
    aldehyde dehydrogenase 1 family member L1
  • Target synonyms
    10-formyltetrahydrofolate dehydrogenase; 10-fTHF; 10-FTHFDH; cytosolic 10-formyltetrahydrofolate dehydrogenase; FDH; FTHFD; MER57B-ALDH1L1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75891
  • Protein Name
    Cytosolic 10-formyltetrahydrofolate dehydrogenase
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDH1L1 using anti-ALDH1L1 antibody (A04615-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1L1 antigen affinity purified polyclonal antibody (Catalog # A04615-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1L1 at approximately 97KD. The expected band size for ALDH1L1 is at 97KD.
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