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ALDH1L1 Antibody (YA2031, Rabbit)

HY-P82286
MedChem Express
TargetALDH1L1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ALDH1L1 Antibody (YA2031, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10840
  • Target name
    ALDH1L1
  • Target description
    aldehyde dehydrogenase 1 family member L1
  • Target synonyms
    10-formyltetrahydrofolate dehydrogenase; 10-fTHF; 10-FTHFDH; cytosolic 10-formyltetrahydrofolate dehydrogenase; FDH; FTHFD; MER57B-ALDH1L1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    ALDH1L1 Antibody (YA2031) is a non-conjugated IgG antibody, targeting ALDH1L1, with a predicted molecular weight of 99 kDa (observed band size: 99 kDa). ALDH1L1 Antibody (YA2031) can be used for WB experiment in mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDH1L1 using anti-ALDH1L1 antibody (A04615-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1L1 antigen affinity purified polyclonal antibody (Catalog # A04615-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1L1 at approximately 97KD. The expected band size for ALDH1L1 is at 97KD.
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