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Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody.
Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody.
Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody.

ALDH2 Antibody

CSB-PA005566
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDH2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID217
  • Target name
    ALDH2
  • Target description
    aldehyde dehydrogenase 2 family member
  • Target synonyms
    acetaldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDM; epididymis secretory sperm binding protein; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05091
  • Protein Name
    Aldehyde dehydrogenase, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Aquaporin 1 using anti-Aquaporin 1 antibody (PB9472). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1 antigen affinity purified polyclonal antibody (Catalog # PB9472) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 56 kDa. The expected band size for Aquaporin 1 is at 56 kDa.
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