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Immunohistochemical staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.

Anti-ALDH2-25ul

HPA051065
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALDH2-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05091
  • Protein Name
    Aldehyde dehydrogenase, mitochondrial
  • Scientific Description
    Rabbit Polyclonal Anti-ALDH2 Antibody against Human aldehyde dehydrogenase 2 family (mitochondrial). Validated for Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aquaporin 1 using anti-Aquaporin 1 antibody (PB9472). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1 antigen affinity purified polyclonal antibody (Catalog # PB9472) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 56 kDa. The expected band size for Aquaporin 1 is at 56 kDa.
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