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ProductsBack/ALDOB Antibody - middle region (OASG00313)

ALDOB Antibody - middle region (OASG00313)

OASG00313
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetALDOB
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ALDOB Antibody - middle region (OASG00313)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID229
  • Target name
    ALDOB
  • Target description
    aldolase, fructose-bisphosphate B
  • Target synonyms
    ALDB, ALDO2, fructose-bisphosphate aldolase B, aldolase 2, aldolase B, fructose-bisphosphatase, aldolase B, fructose-bisphosphate, liver-type aldolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDOB using anti-ALDOB antibody (A03893-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: monkey liver tissue lysates, Lane 9: monkey kidney tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOB antigen affinity purified polyclonal antibody (Catalog # A03893-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDOB at approximately 39KD. The expected band size for ALDOB is at 39KD.
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